Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Constitutive RIBEYE knockout, C57BL/6 background | PMID: 26929012 | ||
| Antibody | CtBP2 (mouse monoclonal) | BD Biosciences | 612044 | 1:200 |
| Antibody | PSD-95 (mouse monoclonal) | Sigma Aldrich | P246-100ul | 1:200 |
| Antibody | Bassoon SAP7f407 (mouse monoclonal) | Abcam | ab82958 | 1:200 |
| Antibody | Bassoon (guinea pig polyclonal) | Synaptic Systems | 141 004 | 1:500 |
| Antibody | RIM2 (rabbit polyclonal) | Synaptic Systems | 140 103 | 1:100 |
| Antibody | Cav1.3 (rabbit polyclonal) | Alomone Labs | ACC 005 | 1:75 or 1:100 |
| Antibody | Piccolino (rabbit polyclonal) | Regus-Leidig et al. (2013) | 1:500 | |
| Antibody | Parvalbumin α (guinea pig polyclonal) | Synaptic Systems | 195 004 | 1:1000 |
| Antibody | Calbindin 28 k (mouse monoclonal) | Swant | 07(F) | 1:500 |
| Antibody | Calretinin (rabbit polyclonal) | Swant | 1893–0114 | 1:1000 |
| Antibody | Alexa Fluor 488 conjugated anti-rabbit (goat polyclonal) | Invitrogen | A11008 | 1:200 |
| Antibody | Alexa Fluor 488 conjugated anti-guinea-pig (goat polyclonal) | Invitrogen | A11004 | 1:200 |
| Antibody | Alexa Fluor 568 conjugated anti-mouse (goat polyclonal) | Invitrogen | A11073 | 1:200 |
| Antibody | Alexa Fluor 647 conjugated anti-rabbit (donkey polyclonal) | Invitrogen | A31573 | 1:200 |
| Antibody | STAR580 conjugated anti-mouse (goat polyclonal) | Abberior | 2-0002-005-1 | 1:200 |
| Antibody | STAR580 conjugated anti-rabbit (goat polyclonal) | Abberior | 2-0012-005-8 | 1:200 |
| Antibody | STAR635p conjugated anti-mouse (goat polyclonal) | Abberior | 2-0002-007-5 | 1:200 |
| Antibody | STAR635p conjugated anti-rabbit (goat polyclonal) | Abberior | 2-0012-007-2 | 1:200 |
| Software, algorithm | Patchmaster or Pulse | http://www.heka.com/products/products_main.html#soft_pm | RRID:SCR_000034 | |
| Software, algorithm | IGOR Pro | http://www.wavemetrics.com/products/igorpro/igorpro.htm | RRID:SCR_000325 | |
| Software, algorithm | Patchers Power Tools | http://www3.mpibpc.mpg.de/groups/neher/index.php?page=software | RRID:SCR_001950 | |
| Software, algorithm | MATLAB | http://www.mathworks.com/products/matlab/ | RRID:SCR_001622 | |
| Software, algorithm | Gatan Microscopy Suite | http://www.gatan.com/products/tem-analysis/gatan-microscopy-suite-software | RRID:SCR_014492 | DigitalMicrograph scripting |
| Software, algorithm | Reconstruct | PMID: 15817063 | ||
| Software, algorithm | Serial-EM | PMID: 16182563 | ||
| Software, algorithm | 3dmod | PMID: 8742726 | ||
| Software, algorithm | IMOD | http://bio3d.colorado.edu/imod | RRID:SCR_003297 | |
| Software, algorithm | Genetic fit algorithm | Sanchez del Rio and Pareschi, 2001 | doi: 10.1117/12.411624 | |
| Software, algorithm | Fiji | http://fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm | Imaris | http://www.bitplane.com/imaris/imaris | RRID:SCR_007370 | |
| Software, algorithm | Excel | microsoft.com/mac/excel | ||
| Software, algorithm | Origin | http://www.originlab.com/index.aspx?go=PRODUCTS/Origin | RRID:SCR_014212 | |
| Software, algorithm | GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | RRID:SCR_015807 | |
| Software, algorithm | Java Statistical Classes library | Bertie, 2002 |