Skip to main content
. 2018 Jan 22;16(1):e2002399. doi: 10.1371/journal.pbio.2002399

Fig 4. A hippo-yki signal is essential for proper planarian regeneration.

Fig 4

(A) Quantification of H3P+ cells after 6 and 48 hRs in hippo (RNAi) animals and corresponding controls; n ≥ 8. (B) Stereomicroscopic view of live control and hippo (RNAi) animals after 5–10 dRs; n = 10. White arrow indicates the smaller blastema. (C) FISH for th+ in control, hpo (RNAi), and yki (RNAi) animals after 5 dRs. Nuclei are stained with DAPI. The corresponding quantification is shown (n ≥ 5). The total number of th+ cells was normalized with respect to the head area (from the anterior tip to the pharynx). Error bars represent standard deviation. Data were analyzed by Student t test. *p < 0.05; **p < 0.01. Data used in the generation of this figure can be found in S1 Data. Scale bars: 500 μm (B); 150 μm (C). dR, days of regeneration; FISH, fluorescent in situ hybridization; gfp, green fluorescent protein; hpo, hippo; hR, hours of regeneration; H3P, phospho-histone-H3-Ser10; RNAi, RNA interference; th+, tyrosine hydroxylase (dopaminergic neuron); yki, yorkie.