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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Adv Mater. 2017 Nov 2;29(47):10.1002/adma.201703969. doi: 10.1002/adma.201703969

Figure 4.

Figure 4

Characterization of in vivo dendritic cell maturation. a–d) Analysis of dendritic cell maturation markers CD40 (a), CD80 (b), CD86 (c), and MHC-II (d) in the draining lymph nodes after administration with CpG-CCNPs and various control formulations, including whole cell lysate with free CpG (WC + fCpG), CCNPs with free CpG (CCNP + fCpG), CCNPs, CpG-NPs, and blank solution (n = 4; mean ± SD). e,f) Concentration of pro-inflammatory cytokines IL-6 (e) and IL-12p40 (f) secreted by immune cells isolated from the draining lymph nodes after vaccination with CpG-CCNPs or various control formulations (n = 4; mean ± SEM). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (compared to CpG-CCNP); one-way ANOVA.