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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Hum Mutat. 2017 Sep 26;38(12):1700–1710. doi: 10.1002/humu.23318

FIGURE 2.

FIGURE 2

p.Arg530Ser and p.Thr924Met expression on the cell surface and in cell lysate and the conditioned medium. (A) Cell surface expression of WT corin and p.Arg530Ser and p.Thr924Met in HEK293 cells analyzed by flow cytometry. Data were from nine independent experiments; **P < 0.001 versus WT. The negative data with a nonspecific IgG control are not shown. Corin protein levels in the cell lysate (B) and conditioned medium (C) from the transfected cells were measured by ELISA. Data were from four independent experiments; **P < 0.01 versus variants of the same time point. (D) Corin fragments in the conditioned medium (CM) (top) and cell lysate (bottom) were analyzed by Western blotting. (E) Illustrations of corin fragments generated by ADAM metalloproteinase or corin autocleavage. (F) Quantitative analysis of 180 (left), 160 (middle), and 100 (right) kDa corin fragments detected in Western blotting. Data were from three independent experiments; P values are indicated. n.s.: not significant

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