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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Hum Mutat. 2017 Sep 26;38(12):1700–1710. doi: 10.1002/humu.23318

FIGURE 5.

FIGURE 5

Analysis of corin zymogen activation in transfected HEK293 cells. (A) Effects of PCSK6 overexpression on the zymogen activation of WT corin, p.Arg530Ser and p.Thr924Met in transfected HEK293 cells were analyzed by Western blotting. (B) The ratio of Corin-p versus Corin bands was quantified. Data were from three independent experiments. (C) Effect of p.Arg530Ser expression on WT corin zymogen activation was analyzed by Western blotting. (D) The ratio of Corin-p versus Corin bands was quantified. Data were from five independent experiments. (E) Dominant negative effect of p.Arg530Ser on WT corin zymogen activation in HEK293 cells transfected with corin-expressing plasmids and a control vector. Corin protein fragments in cell lysates were analyzed by Western blotting. (F) The ratio of Corin-p versus Corin bands was quantified. Data were from three independent experiments. *P < 0.05; **P < 0.01 versus WT; n.s.: not significant

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