Fig. 1.

Ops-H. capsulatum are more efficiently phagocytized by AMs and induced higher amounts of PGE₂ and PGD₂ than did H. capsulatum. A: AMs were incubated with H. capsulatum or Ops-H. capsulatum labeled with FITC (MOI 1:10), and phagocytosis was assessed 2 h later. Data are expressed as average of fluorescence intensity (MFI) from internalized yeast (n = 6). To determine PGD₂ (B) and PGE₂ (C) production, AMs were incubated with H. capsulatum or Ops-H. capsulatum (MOI 1:10) during 2 and 48 h, and the supernatants were collected for PG quantification by immunoassays as described in Materials and Methods (n = 3). * P < 0.05 [H. capsulatum vs. Ops-H. capsulatum (A); noninfected AMs vs. AMs + H. capsulatum or vs Ops-H. capsulatum (B, C)]; # P < 0.05 (48 h AMs + H. capsulatum vs. 2 h H. capsulatum); ## P < 0.05 (48 h AMs + Ops-H. capsulatum vs. 2 h Ops-H. capsulatum); $$ P < 0.05 (48 h AMs + H. capsulatum vs. 48 h Ops-H. capsulatum). One-way ANOVA and Tukey’s multiple comparison tests were used. Data are representative of two independent experiments (±SEM).