Fig. 3.

PGD₂ and PGE₂ have opposite effects on phagocytosis and fungicidal activity of AMs. The effects of pretreating AMs with a specific inhibitor of PGD synthase (HQL-79) or PGE synthase (CAY10526), or soluble PGD₂ or PGE₂ on phagocytosis and fungicidal activity were assessed. AMs were pretreated with HQL-79 (1 μM) or/and PGD₂ (1 μM) (A) or CAY10526 (2 μM) or/and PGE₂ (1 μM) (B), respectively, 30 and 2 min before the addition of Ops-H. capsulatum (MOI 1:10) labeled with FITC. Phagocytosis was assessed 2 h later, and data are expressed as average of fluorescence intensity (MFI) from internalized yeast (n = 6). The fungicidal activity was also evaluated following treatment with HQL-79 or CAY10526 (C) or with PGD₂ or PGE₂ (D). After 48 h, AMs were lysed, and CFU number determined live yeast. Fungicidal activity was expressed as a percentage from total internalized yeast by AMs incubated with vehicle (100%) (n = 4). * P < 0.05 [AMs + Ops-H. capsulatum (in vehicle) vs. other groups]. One-way ANOVA and Tukey’s multiple comparison tests were used. Data are representative of two independent experiments (±SEM).