Fig. 6.

TNF-α, IL-1β, IL-10, H₂O₂, and LTB₄ production and BLT1 receptor expression by infected AMs induced by exogenous PGD₂ and PGE₂. AMs were pretreated with PGD₂ (1 μM) or with PGE₂ (1 μM) for 2 min before infection with Ops-H. capsulatum (MOI 1:10). The supernatants were collected after 48 h of incubation and TNF-α (A), IL-1β (B), IL-10 (C), H₂O₂ (D), and LTB₄ (E) concentrations were determined by immunoassays. F: Expression of BLT1 receptor shown was analyzed by flow cytometry. * P < 0.05 (uninfected AMs vs. other groups); # P < 0.05 (AMs + Ops-H. capsulatum vs. other groups). One-way ANOVA and Tukey’s multiple comparison tests were used. Data are representative of two independent experiments (±SEM).