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UCP1 deletion increases adipose SCD and D6D gene expression and products. Indices of desaturation in the TG fraction of inguinal WAT (A) and epididymal WAT (B) and in the plasma FFA fraction (C) were determined using TLC-GC (n = 6/group). D: Transcript levels of Δ9 desaturase isoforms (Scd1, 2, and 3) and Δ6 desaturase in WAT as measured by qPCR (n = 6/group). E: Immunoblotting of SCD1 and SCD2 in inguinal WAT. Data are expressed as mean ± SEM. *P < 0.05 vs. WT.
