Fig. 5.

Mean chain length and lamellar spacing. A: The mean total chain length (carbon atoms) for the four different sites at two depths excluding the ceramides of the formulation in the calculation. Data were compared using an LMM. B: The correlation between changes in fraction EO ceramides and changes in the mean chain lengths (carbon atoms) at the stripped site due to treatment (amount at the stripped site subtracting the stripped+treated). C: The EO fraction of the samples at the four different sites at two depths. Data were compared using an LMM. D: The difference between the SAXD peak 2 position in the stripped and stripped+treated sites. The differences were tested with a Wilcoxon matched pairs signed rank test. E: The correlation between the SAXD peak 2 (nm) and TEWL (g/m²/h) at the end of the study. Pearson r and P are provided. F: The correlation between the SAXD peak 2 (nm) and the mean chain length (carbon atoms) of all ceramides. Pearson r and P are provided. G. The wavenumber in cm⁻¹ of the stretching peak position. Data were compared using an LMM.