Fig. 5.

ACC inhibition attenuates chemotactic migration. A: HUVECs were seeded onto the porous filter membrane insert of a Boyden chamber. Cells were either left untreated or were pretreated with soraphen A (SorA) (30 μM, 18 h). B: HUVECs were transfected with nontargeting (nt) or ACC1-targeting siRNA and seeded onto the porous filter membrane insert of a Boyden chamber. Forty-two hours after transfection, the Boyden chamber assay was started. A, B: Cells were allowed to migrate toward a chemotactic gradient (20% FBS) for 6 h. Migrated cells were quantified by crystal violet staining. Data are expressed as mean ± SEM (n = 4 for A, n = 3 for B) [*P ≤ 0.05 vs. control (ctrl)]. C: HUVECs were either left untreated or were treated with 30 μM soraphen A. D: HUVECs were transfected with nontargeting (nt) or ACC1-targeting siRNA. Twenty-eight hours after transfection, cells were seeded into chemotaxis chambers. C, D: Chemotactic migration within a 20% FBS gradient was monitored for 20 h. The parameters FMI:Y, directness, accumulated distance, Euclidean distance, and the velocity of cells were determined. Data are expressed as mean ± SEM (n = 3) (*P ≤ 0.05 vs. ctrl or siRNA nt).