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. 2017 Dec 27;59(2):312–329. doi: 10.1194/jlr.M080374

Fig. 8.

Fig. 8.

ASM activation is necessary for glutamate-dependent cellular and mitochondrial ROS generation. A. OLs were treated with 1 mM glutamate with/without 25 μM Reclast, 25 μM desipramine, 5 μM ferrostatin-1, 1 μM PMC, or 15 μM GKT137831 for 3 h and DCF fluorescence was measured. Data are mean ± SE, *P < 0.05 compared with nontreated cells, #P < 0.05 compared with glutamate-treated cells, n = 12. B: Cells were exposed to 1 mM glutamate with/without 25 μM Reclast, 25 μM desipramine, 5 μM ferrostatin-1, 1 μM PMC, or 15 μM GKT137831 for 3 h and MitoSox fluorescence was measured. Control cells were treated with either 25 μM sphingosine or 1 μM Rot as a positive control. #P < 0.05 compared with nontreated cells, *P < 0.05 compared with glutamate-treated cells, n = 12. C: OLs were treated with 1 mM glutamate with/without 25 μM Reclast for 3 h and MitoSox fluorescence (MSF) as well as phase contrast images were captured using confocal microscopy.