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. 2017 Dec 11;59(2):330–338. doi: 10.1194/jlr.M080382

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Fig. 2. — HDAC5 interacts with PPARα. A: Immunoblot showing amounts of endogenous PPARα recovered from immunoprecipitation of endogenous HDAC5 prepared from primary hepatocytes. B: Interaction between Flag-tagged HDAC5 and HA-tagged PPARα in HEK293T cells. C: Interaction between Flag-tagged HDAC5 and HA-tagged PPARα or HA-tagged RXRα in HEK293T cells. D: Interaction between Flag-tagged HDAC5 and HA-tagged PPARα in HEK293T cells exposed to FSK (10 μM, 1 h). E: Interaction between Flag-tagged HDAC5 or HDAC5 2SA and HA-tagged PPARα in HEK293T cells. F: Schematic diagram of the structure of HDAC5 and its truncation mutants. G-I: Immunoblots showing effects of mutants in HDAC5 on its association with PPARα in HEK293T cells. IB, immunoblot; IP, immunoprecipitation.