Figure 4.

Enhanced cWnt signaling has high expression levels of BMP3 in the mice. OBs from heterozygous DKK1‐knockout mice or wild‐type littermates (WT) were treated with OB differentiation medium. The messenger RNA levels of BMP3 (A) were determined by qPCR on 0, 7, or 14 days (A). After injection of 0.75 mg·L⁻¹ control vehicle or BIO on three times, the messenger RNA levels of BMP3 and Axin2 were determined by qPCR (A–C) and protein levels of BMP3, β‐catenin, and β‐actin were assessed by western blot analysis (D). The data are expressed as the mean ± SD (n = 3). **P < 0.01 versus wild‐type (A) or control vehicle treatment (B, C).