Table 1. Primer sequences of SNP for EZH2 and SMYD3.
SNP | Primer sequence | Temperature | Time | Circle |
---|---|---|---|---|
EZH2rs12670401 | F: 5′-ACGTTGGATGTACTTGGTTTAGATTGCCTG-3′ | 66°C | 45 s | 30 |
R: 5′-ACGTTGGATGATGGACTAGGTGGTTCAAAC-3′ | ||||
EZHrs6464926 | F: 5′-ACGTTGGATGAGAATTGCCAGGAGATCTCT-3′ | 58°C | 45 s | 40 |
R: 5′-ACGTTGGATGTTCCTTCATTGCCTGTTGCC-3′ | ||||
SMYD3VNTR | F: 5′-CGCCTGTCTTCTGCGCAGTCG-3′ | 56.5°C | 45 s | 40 |
R: 5′-CACCTTCAGCGGCTCCATCCTC-3 |
Abbreviations: F, forward; R, reverse; SNP, single nucleotide polymerase.