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. 2018 Feb 2;38(1):BSR20170656. doi: 10.1042/BSR20170656

Table 1. Primer sequences of SNP for EZH2 and SMYD3.

SNP Primer sequence Temperature Time Circle
EZH2rs12670401 F: 5′-ACGTTGGATGTACTTGGTTTAGATTGCCTG-3′ 66°C 45 s 30
R: 5′-ACGTTGGATGATGGACTAGGTGGTTCAAAC-3′
EZHrs6464926 F: 5′-ACGTTGGATGAGAATTGCCAGGAGATCTCT-3′ 58°C 45 s 40
R: 5′-ACGTTGGATGTTCCTTCATTGCCTGTTGCC-3′
SMYD3VNTR F: 5′-CGCCTGTCTTCTGCGCAGTCG-3′ 56.5°C 45 s 40
R: 5′-CACCTTCAGCGGCTCCATCCTC-3

Abbreviations: F, forward; R, reverse; SNP, single nucleotide polymerase.