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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Invest Dermatol. 2017 Aug 31;138(2):316–324. doi: 10.1016/j.jid.2017.07.842

Fig. 5. Supernatants derived from activated prTh17 clones are bactericidal against P. acnes.

Fig. 5

CFU assay results for P. acnes strain HL096PA1 after 3 h incubation with (a) prTh17 clone PH-1 supernatants, (b) LL-37 (0.1-100μm), and (c) paTh17 clone PA-1 supernatants. Data shows average CFU from three independent experiments (n=3), error bars are mean ± SD. (d-f) Representative transmission electron microscopy (TEM) micrographs of (d) P. acnes strain HL096PA1 after 3 h incubation with activated clone PH-1supernatants, (e) LL-37, and (f) activated clone PA-1supernatants. TEM were imaged at 48K. Compared with the activated PA-1 supernatants (1:2 dilution and neat), the bacteria exposed to activated PH-1 supernatants (1:2 dilution and neat) and LL-37 (50μM and 100μM) exhibit cellular differences indicative of stresses on the membrane. Scale bar = 0.5μm.