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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biochimie. 2017 Sep 11;145:125–130. doi: 10.1016/j.biochi.2017.09.006

Figure 4.

Figure 4

Detection of PD-L1 expression on human pancreatic tumor tissue. Sections of human pancreatic ductal adenocarcinoma tissue were incubated with XA-PDL1-82 (25 nM) or PD-L1 antibody (4.8 μg/ml), followed by streptavidin FITC (green) or Goat Anti-Rabbit IgG (Alexa Fluor® 647) secondary antibody at 1/1000 dilution (red). Hoechst 33342 was used to stain the cell nuclei (blue). An IgG isotype was used as negative control. Expression level of PD-L1 was determined by fluorescence intensity of FITC or AF647.