Figure 1. Effects of TLR stimulation on systemic expression of and humoral immune responses to hFIX as a function of time after muscle-directed gene transfer.
Comparison of systemic hFIX antigen (A) and IgG1 anti-hFIX (B) levels in C57Bl/6 mice after intramuscular co-injection of 1011vg AAV1-hFIX and 50 µg of indicated TLR agonist. (Data represent averages +/−SEM for n=3–4 mice per cohort; *P<0.05 AAV1+TLR9 v. AAV1 only). Experiement was repeated at least twice.