Figure 4. TLR9 stimulation upregulates monocyte-derived dendritic cells (moDCs).

(A–B) C57Bl/6 mice were co-injected with vector and indicated TLR agonist. Twenty-four hours later splenocytes were isolated and analyzed by flow cytometry (A, gating scheme) for frequency of CD11b⁺CD11c⁺CD64⁺ moDC cells (n=3/group) in popliteal and inguinal lymph nodes (B) and spleen (C). Expression of the co-stimulatory molecule CD86 on CD11c^hi is shown as mean fluorescence intensity (MFI) for lymph nodes (D) and spleen (E). Data are average ± SEM, with *P<0.05 and **P<0.01.