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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Invest Dermatol. 2017 Sep 18;138(2):444–451. doi: 10.1016/j.jid.2017.09.004

Figure 3.

Figure 3

α9β1 suppresses α3β1-dependent induction of LNγ2 processing and mTLD/BMP-1 expression. (a,b) ECM fractions or (c,d) whole cell lysates were collected from MK cell variants of the indicated integrin composition, which were cultured in high calcium to promote α3β1-dependent LNγ2 processing, as described (Longmate et al., 2014). (a) Representative immunoblots of matrix preparations using anti-LNγ2. The unprocessed (155 kD) and processed (105 kD) forms of LNγ2 are indicated. (b) Quantification of processed LNγ2 as a proportion of total LNγ2, normalized to the daily mean to account for variability by day. (c) Representative immunoblots for mTLD/BMP-1 and ERK. (d) Quantification of relative mTLD/BMP-1 protein, normalized to ERK. Data are mean ± SEM; n≥3; 1-way ANOVA; Newman-Keuls multiple comparison, *P<0.05.