Table 3.

Influence of RS8070345, smoking, and age on DNA methylation in the VMP1/MIR21 locus in healthy individuals.

CpG probea	Genea	RS8070345b (N = 717)		Current smokerc (N = 994)		Past smokerc (N = 994)		Agec (N = 994)
		Coeff	p-val	Coeff	p-val	Coeff	p-val	Coeff	p-val
cg16936953	VMP1	–0.0106	0.049	–0.0098	0.34	–0.0060	0.37	–0.0010	5E-07
cg12054453	VMP1	–0.0170	1E-04	–0.0158	0.07	–0.0120	0.031	–0.0005	4E-03
cg01409343	VMP1	–0.0046	0.43	0.0011	0.92	0.0002	0.97	–0.0011	2E-07
cg18942579	VMP1	–0.0086	0.13	0.0000	1.00	0.0020	0.78	–0.0011	3E-07
cg02782634	VMP1	0.0016	0.78	–0.0065	0.55	0.0007	0.92	–0.0010	5E-07
cg14032089	MIR21	0.0057	0.33	–0.0041	0.71	0.0019	0.79	–0.0009	2E-05
cg27023597	MIR21	–0.0058	0.34	–0.0126	0.28	0.0002	0.98	–0.0010	5E-06
cg04276626	MIR21	0.0032	0.58	–0.0057	0.59	0.0028	0.68	–0.0004	0.06
cg02515217	MIR21	0.0016	0.82	0.0022	0.85	–0.0001	0.99	–0.0012	3E-07
cg15759721	MIR21	0.0048	0.42	–0.0017	0.88	0.0019	0.79	–0.0010	1E-06
cg07181702	MIR21	0.0034	0.57	0.0011	0.92	0.0014	0.85	–0.0011	4E-07

^aCpG probe ID and annotated gene names from 450K arrays.

^bCoefficients (Coeff) and p-values (p-val) from a linear mixed-effects model with the methylation β-score at each CpG site as the outcome and the following predictors: genotype at RS8070345, age, sex, study site, current smoking, body mass index (BMI), principal components capturing T-cell purity, and family, as previously described.¹⁹ p-val <0.05 are indicated in bold.

^cCoefficients (Coeff) and p-values (p-val) from a linear regression model with the methylation β-score at each CpG site as the outcome and the following predictors: age, current and past smoking, BMI, and principal components capturing T-cell purity, as previously described.¹⁹ p-val <0.05 are indicated in bold.