Fig. 7.

Validation of the influence of plasminogen (PLG), antithrombin III (AT III), fibrinogen gamma chain (FGG), and transthyretin (TTR) on HT-22 cell viability by small interfering RNA (siRNA) treatment. (a) PLG, AT III, FGG, and TTR were knocked down in cells. Then targeted temperature management (TTM) was applied and CellTiter 96 AQueous One solution Cell Proliferation Assay was used to measure cell viability. (b) Effects of FGG knockdown and TTM on the expression of FGG and proliferating cell nuclear antigen (PCNA). (c) Effects of AT III knockdown and TTM on the expression of AT III and PCNA. (d) Effects of PLG knock down and TTM on the expression of PLG and PCNA. *p < 0.05 vs the control group; ^# p < 0.05 vs the traumatic brain injury (TBI) group; ^& p < 0.05 vs the TBI + siRNA knockdown group. CIC = cell injury controller