Inhibition of Rac blocks pro-inflammation cells recruitment in colon tissue. A, B. Flow cytometry analysis of CD8+ T cells, CD4+ T cells, Ly6G+CD11b+ MDSC cells, F4/80+ macrophages and NK1.1+ NK cells in colonic tissue of chronic colitis mice after DSS feeding for 7 days and 14 days. n=3, **, P<0.01. C. Immunofluorescent staining analysis of iNOS and Ly6G expression in colonic tissue of chronic mice. Scale bar = 100 μm. D. The number of iNOS and Ly6G positive cells were counted and analyzed. n=4, **, P<0.01. E. Immunofluorescent staining analysis of iNOS and F4/80 expression in colonic tissue of chronic mice. Scale bar = 100 μm. F. The number of iNOS and F4/80 positive cells were counted and analyzed. n=4, **, P<0.01.