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. 2018 Jan 1;8(1):192–206.

Figure 5.

Figure 5

CNALPTC1 sponges and down-regulates miR-30 family. A. Schematic outlining the predicted binding site of miR-30 family on CNALPTC1. B. The specific bindings of miR-30 family to CNALPTC1 or miR-30 binding site mutated CNALPTC1 (CNALPTC1-mut) were determined by MS2 based RIP assays, followed by qRT-PCR. C. TPC-1 cell lysates were incubated with biotin-labeled CNALPTC1 or CNALPTC1-mut; after pull-down, microRNAs were extracted and determined by qRT-PCR. D. CNALPTC1 or miR-30 binding site mutated CNALPTC1 (CNALPTC1-mut) overexpression plasmids were transfected into TPC-1 cells. Forty-eight hours later, miR-30 family expression levels were determined by qRT-PCR. E. Expression levels of miR-30 family were determined by qRT-PCR in CNALPTC1 stably depleted and control TPC-1 cells. Results are shown as mean ± s.d. of 3 independent experiments. **P < 0.01, ***P < 0.001 by Student’s t-test. RIP, RNA Immunoprecipitation; qRT-PCR, quantitative real-time PCR.