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. 2018 Jan 1;8(1):192–206.

Figure 7.

Figure 7

CNALPTC1 exerts oncogenic activity in PTC via sponging miR-30 family. A. After transfection of CNALPTC1 or CNALPTC1-mut overexpression plasmids, or co-transfection of CNALPTC1 overexpression plasmids and miR-30 mimics mix into TPC-1 cells, cell proliferation was determined by CCK-8 assays. B. After transfection of CNALPTC1 or CNALPTC1-mut overexpression plasmids, or co-transfection of CNALPTC1 overexpression plasmids and miR-30 mimics mix into TPC-1 cells, cell proliferation was determined by EdU incorporation assays. The red colour indicates EdU-positive nuclei. Scale bars = 100 µm. C. After transfection of CNALPTC1 or CNALPTC1-mut overexpression plasmids, or co-transfection of CNALPTC1 overexpression plasmids and miR-30 mimics mix into TPC-1 cells, cell apoptosis was determined by TUNEL assays. The green colour indicates TUNEL-positive or apoptotic cells. Scale bars = 100 µm. D. After transfection of CNALPTC1 or CNALPTC1-mut overexpression plasmids, or co-transfection of CNALPTC1 overexpression plasmids and miR-30 mimics mix into TPC-1 cells, cell migration was determined by transwell assays. Represent images are shown. Scale bars = 100 µm. Results are shown as mean ± s.d. of 3 independent experiments. *P < 0.05, **P < 0.01, ns, not significant, by Student’s t-test. PTC, papillary thyroid cancer; CCK-8, Cell Counting Kit-8; EdU, Ethynyl deoxyuridine; TUNEL, TdT-mediated dUTP nick end labeling.