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. 2018 Feb 1;8:2148. doi: 10.1038/s41598-018-19766-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic analysis and expression profiling of Brassinosteroid responsive genes in EcbZIP17-T1 transgenic tobacco plants. (a) Representative image after 115 days at normal growth condition. (b) Graph chart representation of plant height, leaf width, stem girth, internode length, pod number and total seed weight of WT and transgenic lines at flowering stage. (c) Expression pattern of Brassinosteroid responsive genes in seedling and flowering stage of WT and transgenic lines. Data represents mean ± SE of three replicates (n = 3). According to one way ANOVA (P < 0.05) different lower case letters above the error bars indicate significant difference in expression of transgenic lines over WT after normalization. L25 was used as internal reference gene for normalization.