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. 2018 Feb 1;8:2105. doi: 10.1038/s41598-018-20512-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal expressions of nerve growth factor (NGF) immunoreactivity in the bladder of control, STZ-induced diabetic rats (DM) and diabetic rats + hAFSCs transplantation (DM + hAFSCs). The immunoreactivity of NGF can be seen in control rats (A), diabetic rats (B), and diabetic rats + hAFSCs transplantation (C) at 4 weeks, and in control rats (D), diabetic rats (E), and diabetic rats + hAFSCs transplantation (F) at 12 weeks. When compared to the controls, the expressions of NGF in the diabetic rats are significantly decreased, but can be recovered to near the control level after hAFSCs transplantation. OD = optical density. Bar indicates 40 μm. *P < 0.05 vs. control group. N = 10 in each time point.