Table 1.

Biophysicals properties of WT, R1451C and R1451L channels at 22 °C.

	WT		R1451C		R1451L
	Mean ± sem	n	Mean ± sem	n	Mean ± sem	n
Steady-state activation		9		8		15
V1/2	−27.5 ± 1.8		−28.8 ± 2.8		−30.7 ± 2.5
kv	−5.8 ± 0.3		−7.6 ± 0.5**		−7.9 ± 0.3***
Steady-state inactivation		7		8		9
V1/2	−72.2 ± 1.4		−78.2 ± 2.1*		−91.8 ± 3.3***
kv	4.2 ± 0.1		5.9 ± 0.2***		9.4±0.3***
Recovery from fast inactivation		5		8		6
τ	1.1 ± 0.1		1.1 ± 0.1		1.3 ± 0.1
Onset of slow inactivation		10		7		8
τfast	39 ± 9.5		36 ± 11		38 ± 16
Afast	6.4 ± 1.5		10 ± 1.4		7.9 ± 0.8
τslow	3810 ± 465		4193 ± 376		3085 ± 345
Recovery from slow inactivation (4-s cond. pulse)		7		6		7
τfast	3.3 ± 0.5		4.2 ± 1.8		2 ± 0.2
Afast	47 ± 5.4		43 ± 4.9		41 ± 5.5
τslow	243 ± 43		291 ± 79		420 ± 105
Recovery from slow inactivation (30-s cond. pulse)		8		5		7
τfast	24 ± 12		59 ± 16		162 ± 46**
Afast	46 ± 6.5		54 ± 12		41 ± 8.9
τslow	382 ± 64		726 ± 97**		1978 ± 206***

V_1/2, midpoint for activation or inactivation (mV); k, slope factor for activation or inactivation; τ, time constant (ms); A, fraction of the τ components (%); n, number of cells. Values are means ± sem, *P < 0.05, **P < 0.001, ***P < 0.001, data were significantly different for mutant channels when compared to WT at 22 °C.