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. 2018 Feb 1;8:2135. doi: 10.1038/s41598-018-20566-z

Figure 3.

Figure 3

Comparison of T-cell subsets. Flow cytometric analysis of T-cell subset was performed using blood samples collected before treatment and at 2, 3, 5, 6, 7, and 8 weeks after treatment. For analysis of T-cell populations peripheral blood mononuclear cells (PBMCs) were stained with antibodies against CD45, CD3, CD4, CD8, CD28, and CD95. For analysis of regulatory T cells, PBMCs were stained with antibodies against CD45, CD4, CD25, and FoxP3. Data are expressed as the mean ± SEM (n = 4 per group). The means of groups at each time points were compared using student t-tests. *Significant (p < 0.05) differences from the control group are indicated.