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. 2018 Feb 1;8:2135. doi: 10.1038/s41598-018-20566-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of B-cell subsets. Flow cytometric analysis for B-cell subsets was performed using blood samples collected before treatment and at 2, 3, 5, 6, 7, and 8 weeks after treatment. PBMCs were stained with antibodies against CD45, CD3, CD20, CD27, CD21, IgD, and IgM. Data are expressed as the mean ± SEM (n = 4 per group). The means of groups at each time points were compared using student t-tests. *Significant (p < 0.05) differences from the control group are indicated.