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. 2018 Feb 1;8:2071. doi: 10.1038/s41598-018-20311-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BS induced ROS and apoptosis associated with DNA damage in A549 cells. (a) Panel of fluorescence microscopic images showed comet like structure upon BS treatment after 72 h incubation time and this effect was reversed by addition of NAC (10 mM). (b) Nuclear morphological changes induced by BS were assessed by DAPI staining and visualized in fluorescence microscopy at a magnification of 200×. NAC reversed the nuclear morphological changes induced by BS. (c) Representative histogram of different comet factors on BS exposure was done using ImageJ software. *P < 0.05, **P < 0.01, ***P < 0.0001, significantly different when compared with control, ^#P < 0.05 for BS versus NAC + BS.