Skip to main content
. 2018 Feb 1;8:2097. doi: 10.1038/s41598-018-20146-1

Figure 6.

Figure 6

Paxillin regulates accumulation of acetylated tubulin in RasV12-transformed cells surrounded by normal cells. (a) Effect of paxillin-knockdown on accumulation of acetylated α-tubulin. MDCK-pTR GFP-RasV12 cells or MDCK-pTR GFP-RasV12 paxillin-shRNA1 cells were mixed with normal MDCK cells on collagen gels. Cells were fixed after 16 h incubation with tetracycline and stained with anti-acetylated α-tubulin (red) and anti-paxillin (grey) antibodies and Hoechst (blue). Scale bar, 10 μM. (b) Quantification of (a). Data are mean ± SD from three independent experiments. *P < 0.01; n ≧ 30 cells for each experimental condition. Values are expressed as a ratio relative to paxillin-shRNA1 (-).