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. 2018 Feb 1;8:2127. doi: 10.1038/s41598-018-20557-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Model of RecF with dsDNA. BLI analysis of (A)TTERecF and (C)TTERecF-ATP dimer interaction with ssDNA at 25 °C. BLI analysis of (B) TTERecF and (D) TTERecF-ATP dimer interaction with dsDNA at 25 °C. Sensorgrams are shown for different concentrations of TTERecF monomer or TTERecF-ATP dimer injected over 21 mer ssDNA or 21-mer dsDNA coupled streptavidin biosensors. The apparent K_D values were calculated from the kinetic K_D (M) = k_d/k_a. (E) Cartoon representations of Rad50-dsDNA (PDB:5DNY). (F) Speculated structural model of RecF with dsDNA.