Table 1.
Primer sequences used for RT-qPCR.
| Gene | NCBI reference sequence | Forward primer | Reverse primer |
|---|---|---|---|
| Bmp4 | NM_012827.2 | TGAGGGATCTTTACCGGCTC | CTCCAGATGTTCTTCGTGATGG |
| Cnp | NM_012809.2 | CAACAGGATGTGGTGAGGAG | CCTGCTCATTAAGCACCACC |
| Eef1a1 | NM_175838.1 | GATGGCCCCAAATTCTTGAAG | GGACCATGTCAACAATTGCAG |
| Gpr17 | NM_001071777.1 | CTTCTCTGGCAATCACTGGC | ACTTGACTGGGTGCACAATG |
| Hes1 | NM_024360.3 | GAAAGATAGCTCCCGGCATTC | GTACTTCCCCAACACGCTC |
| Hes5 | NM_024383.1 | TGAAGCACAGCAAAGCCTTC | ACGAGTAACCCTCGCTGTAG |
| Hmbs | NM_013168.2 | CCGAGCCAAGCACCAGGAT | CTCCTTCCAGGTGCCTCAGA |
| Id2 | NM_013060.3 | CCAGAGACCTGGACAGAACC | GAATTCAGACGCCTGCAAGGAC |
| Ifngr1 | NM_053783.1 | CTAAGTCCTTGCTCTCTGTGG | GTCACTGTGGACAAGTGCTC |
| Ifngr2 | NM_001108313.1 | TACAGCTACGTCGATGGCTC | GCGCAGGAAGACTGTGTATG |
| Itpr2 | NM_031046.3 | GCTACACAACAACCGCAAAC | TAGTCCAGAAACCTCGGCTC |
| Lpar1 | NM_053936.3 | TGCCCTTTGGCCAGGCTTTC | AGTTTGGAGCGATGAAGAGGC |
| Mbp | NM_001025289.1 | AGAACTACCCACTACGGCTC | GGTGTACGAGGTGTCACAATG |
| Myrf | NM_001170487.1 | AGCCCTCCAATATAGACACCAG | TCAGGGAAGCAGAGGTCAG |
| Neu4 | NM_001108234.1 | CTATTGCTTTACGCTCCCTGG | CGGTTCTCTTGGGAACATGC |
| Nkx6–2 | NM_001107558.2 | AGAGCCAGGTGAAGGTGTG | TCTTTTTAGCCGACGCCATC |
| Opalin | NM_001017386.1 | TGAGCCCATCGAGGAGACTG | TGTGACCTTCTTGAGCACCTC |
| Pdgfra | NM_012802.1 | AAGATGCTCAAACCCACAGC | ACAATGTTCAGATGCGGTCC |
| Sox6 | NM_001024751.1 | ATGCCTCCGCTCATGATCCC | GGGGTAGTTATCACCTGGCTTG |
| Sox9 | NM_080403.1 | CAAGCTCTGGAGACTGCTG | GCCCATTCTTCACCGACTTC |
| Tcf7l2 | NM_001191052.1 | CATTTTCAATCCGGCAGCAC | CTTCCTGCTTGGACATCGAG |
| Tnfrsf1a | NM_013091.1 | TGTCCCCAGGGAAAGTATGC | CCAAGTAGGTTCCTTTGTGGC |
| Tnfrsf1b | NM_130426.4 | ATTGAACCAAGCATCACGGG | GCAGGAGGGCTTCTTTTTCC |