Fig. 1.

Growth of WT, GFP, J03, and J03-derivative strains in inducing culture conditions (CSL➔HMM). a β-glucosidase activity in culture supernatant as measured using the described pNPG assay: addition of ionic liquid [C₂mim]OAc to reactions allows for distinction between native and heterologous enzymes. Enzyme activity (millimolar equivalents) was normalized to dry weight of fungal biomass (g). The addition of ionic liquid to the reactions allows for distinction between native and heterologous enzyme activity. b Total secreted protein as measured by Bradford assay (μg/μL) and normalized to dry weight of fungal biomass (g). Data drawn from biological replicates (n = 3); error bars indicate standard deviation