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. 2018 Feb 1;8:2093. doi: 10.1038/s41598-018-20469-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of wild-type (Wt) and A-CNP transgenic (Tg) mice fed a high-fat diet (HFD). (A) Schematic representation of the A-CNP Tg transgene, (B) body weight, (C) food intake, (D) macroscopic appearance, and (E) naso-anal length of Wt and A-CNP Tg mice on HFD. (F) CNP and (G) guanylyl cyclase B (GCB) mRNA expression in adipose tissue (epididymal, mesenteric, and inguinal white adipose tissue [EpiWAT, MesWAT, and IngWAT, respectively] and brown adipose tissue [BAT]) from Wt and A-CNP Tg mice fed HFD at 10 weeks. pA, polyA tail. a.u., arbitrary units (copy number of gene of interest/copy number of reference gene [ribosomal protein 36B4]). The data are presented as means ± SEM. n = 16–17 (B,C and E); n = 10 (F and G); (B) ^*P < 0.05 for standard (STD)-fed group vs HFD-fed group; (E and F) ^*P < 0.05.