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. 2018 Feb 1;8:2134. doi: 10.1038/s41598-018-20571-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KP-10 induces osteogenic gene expressions in C3H10T1/2 cells. (A and B) RT-PCR were performed using total RNA isolated from C3H10T1/2 cells treated with 5 or 50 μM KP-10 for 2 days (A), 50 μM KP-10 for 1 or 2 days (B). (C) Real-time PCR were performed using total RNA isolated cells treated with 50 μM of KP-10 for 2 days. *p < 0.05; **p < 0.01 and ***p < 0.005 compared with the untreated control. Data represent the mean ± SEM of three individual experiments. (D) Effects of KP-10 on ALP. C3H10T1/2 cells were treated with 50 μM KP-10 in the presence of ascorbic acid and β-glycerophosphate (AA + β-GP) for 4days. (E) ARS staining was detected after treating cell with 50 μM KP-10 for 20 days.