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. 2018 Feb 1;8:2035. doi: 10.1038/s41598-018-20400-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VIP 3′UTR as a target of miRNA miR-205-5p. (a) Transfection with empty PsiCheck2 vector and PsiCheck2 vector-3′UTR inserts with T or C allele for the rs688136 respectively. Data are represented as the mean ± SEM. n = 10 each group. n.s: non-significant. (b) Bioinformatic prediction of hsa-miR-205-5p recognition elements on human VIP 3′UTR. Analyses performed with the TargetScanHuman algorithm. Letter U highlighted in bold corresponds to rs688136 position. Dotted box indicates pairing region. (c) Co-transfection with PsiCheck2 vector-3′UTR inserts with T or C allele and mimic miRNAs miR-205-5p and miR-39-3p (control). Data are represented as the mean ± SEM. n = 8 each group. RLU: relative luminescence unit. Statistical significance in panels a and c was established using Mann-Whitney test for a p-value < 0.05. Each experiment was made in duplicate.