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. 2018 Feb 1;8:2170. doi: 10.1038/s41598-018-19902-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Affinity chromatography purification of PcCDA catalytic domain. (A) Elution profile monitored by absorbance at 280 nm. (B) SDS PAGE analysis of fractions: Lane 1, sample after refolding by dialysis. Lanes 2 and 3, sample after centrifugation and filtered (0.45 μm) loaded into the column. Lane 4, flow-through from the column. Lane 5, eluted fraction with 2.5 mM d-Desthiobiotin after concentration by ultrafiltration. Arrow indicates column elution using d-Desthiobiotin. Arrowhead points to bands with the expected size (26.8 kDa) for PcCDA catalytic domain.