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. 2018 Feb 1;8:2170. doi: 10.1038/s41598-018-19902-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MS/MS spectrum of the mono-deacetylated product (ADAAA) from the PcCDA reaction with (GlcNAc)₅. The reaction mixture contained mainly mono-deacetylated product and traces of di-deacetylated product. The mixture was subjected to the procedure reported in⁴⁰. After reducing-end labelling with H₂(¹⁸O), the sample was analyzed by UHPLC-ESI-MS². Fragmentation spectrum of the mono-deacetylated product (A₄D₁¹⁸O): b-ions are fragments from the non-reducing end, and y-ions are fragments from reducing end with the ¹⁸O label.