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. 2018 Feb 1;8:2194. doi: 10.1038/s41598-018-19854-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Graphical presentation of different groups of mice for in vivo chemoprotection study. (B) Effect of MUS on CBDCA-induced cytotoxicity and clastogenic effect in murine bone marrow cells. Photomicrographs after TUNEL assay were taken at 200× magnification. Brightly-stained nucleus represents an apoptotic cell, whereas, unstained cells represent non-apoptotic cells. (C) Inhibition of CBDCA-induced cell death of murine bone marrow cells by MUS. After treatment completion, murine bone marrow cells were isolated and stained with annexin-V and PI as described in Methods and analyzed by flow cytometry. The figure is a representative profile of at least three experiments in duplicate.