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. 2018 Feb 1;8:2194. doi: 10.1038/s41598-018-19854-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MUS on CBDCA-induced cytotoxicity and clastogenic effect in murine bone marrow cells. (A) Representative photomicrograph (taken at 400 × magnification) of cell proliferation, where proliferating cells are BCIP/NBT stained and non-proliferating cells are unstained. (A1) Representative photomicrograph for the formation of micronuclei (indicated by broken arrow) after CBDCA treatment (1000X). (B) Effect of MUS on CBDCA-induced DNA damage in murine bone marrow cells. Photomicrographs after comet assay were taken at 400 × magnification. (C) Photomicrograph of agarose gel electrophoresis of genomic DNA. (D) Representative photomicrograph of chromosomal aberrations in a single metaphase plate taken at 1000 × magnification. Different anomalies like break [B], sister chromatid union [SCU], fragmentation [F], gap [G] and ring [R] are indicated.