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. 2018 Feb 1;8:2088. doi: 10.1038/s41598-018-20264-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rtPCR PEPCK expression measurements in liver (A) and kidney (B) of age and sex matched WT (Fa/Fa) (n = 4), obese Zucker (fa/fa) (n = 9), and WT-STZ (n = 6) rats. The fold changes were measured relative to WT (Fa/Fa) and calculated with 2^−ΔCt method. The data were normalized to β-actin. All data represent mean+/−S.E.M. from three independent experiments. Statistical analysis was performed by one-way ANOVA with Tukey correction for multiple comparisons. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).