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. 2018 Feb 1;8:2185. doi: 10.1038/s41598-018-20582-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Supernatant from PSMα3 treated neutrophils activates platelets. 10,000 isolated PMNLs/µL were treated with 60 µg/mL PSMα3 for 1 h then centrifuged. Platelets were subsequently incubated with the neutrophil supernatant and fibrinogen-FITC binding was analysed by flow cytometric analysis. Statistical significance was analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons test (*p < 0.05; **p < 0.01).