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. 2018 Feb 1;8:2146. doi: 10.1038/s41598-018-20517-8

Figure 4.

Figure 4

Functional heterologous receptor assay of CHO-K1 aequorin cells transiently expressing the AedaeACPR-I. (a) Dose dependent effect on the bioluminescence response (mean 0–15 s) after the addition of between 10−12–10−5 M doses of A. aegypti ACP, AKH and CRZ peptides. Luminescence is normalized to the BSA control and plotted relative to the maximal response (10−5 M). The EC50 for AedaeACP is 1.025 × 10−8 M. No receptor activation was detected when challenged with AedaeAKH and AedaeCRZ or AedaeCAPA1 and AedaePK1 (not shown). (b) Kinetics of the bioluminescence response measured between 0–5 s, 6–10 s, and 11–20 s time intervals, following the addition of 1 × 10−6 M of the above peptides normalized to vehicle control (BSA media). Luminescence is normalized to the BSA control and plotted relative to the maximal response (10−5 M). Data represent the mean ± standard error (n = 4).