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. 2018 Feb 1;8:2146. doi: 10.1038/s41598-018-20517-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcript expression pattern of ACPR-I and ACP during post-embryonic development and in specific tissues of four-day old adult A. aegypti. Temporal expression of ACPR-I (a) and ACP (b) transcript is analyzed across developmental stages of the mosquito with expression shown relative to transcript levels in pupa. Spatial expression is analyzed in various tissues from four-day old adult males, ACPR-I (c) and ACP (d), and females, ACPR-I (e) and ACP (f) with transcript abundance shown relative to levels in whole body adult mosquitoes. Abbreviations: thoracic ganglia (TG), abdominal ganglia (AG), midgut (MG), Malpighian tubules (MT), hindgut (HG), ovaries (OVA), testes (TE), accessory reproductive tissue (ART). Data represent mean ± standard error, *denotes significance (p < 0.05) as determined by a one-way ANOVA, Dunnett’s multiple comparisons test.