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. 2018 Feb 1;8:2101. doi: 10.1038/s41598-018-20525-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FcRn binding properties of aflibercept. Binding of titrated amounts (1000.0–0.5 ng/ml) aflibercept from (A) D0 and D7, and (B) D0 and D28 to GST-tagged human FcRn at pH 6.0 and 7.4 in ELISA. (C) Comparison of aflibercept (111 nM) binding to human FcRn at D0 and D7, and (D) D0 and D28 at pH 6.0. The data represent mean ± S.D of duplicates from one independent experiment (n = 6). Unpaired Student’s t-test was used for statistical calculations.