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. 2018 Feb 1;8:2109. doi: 10.1038/s41598-018-20444-8

Figure 4.

Figure 4

IL-13 increases ASL viscosity. Differentiated HBE cells were cultured ± IL-13 for 3 days. The apical surface was rinsed and labeled with non-absorbable fluorescent dextran. The following day the ASL height was measured and FRAP was performed. (a) Representative images of ASL height with and without IL-13. (b) The ASL height is not significantly changed by IL-13 in HBE cells as measured by confocal microscopy. Data shown is mean ASL height ± SEM (µm) of experimental replicates from 9 tissue donors, each replicate with 4–6 culture filters, NS by unpaired Student’s t-test. (c) Representative FRAP tracing demonstrating the relative signal recovery in HBE cell culture with and without IL-13. (d) ASL viscosity is increased by IL-13 in HBE cell culture as measured by FRAP. Data shown represents mean τASL/τsaline ± SEM of replicates from 9 tissue donors, each replicate with 4–6 cultures, *p < 0.0001 by unpaired Student’s t-test. Control conditions are represented in black, IL-13 treated conditions are represented in red.