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. 2018 Feb 1;8:2102. doi: 10.1038/s41598-018-20283-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential Scanning Fluorimetry based high-throughput ligand screening of PA2652-LBD. Shown are Tm changes for each of the 95 compounds present in the Biolog PM1 compound array of carbon sources with respect to the Tm of the ligand-free protein of 45.5 °C. The dashed line indicates the threshold of 2 °C for significant hits.