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. 2018 Feb 1;8:2117. doi: 10.1038/s41598-018-20331-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mascot annotated O-glycopeptide MS2 spectra of fetuin using stepped NCE values. Bovine fetuin was digested with trypsin, analyzed by LC-MS using the stepped NCE function (15, 25 and 35) and searched against the custom O-glycoprotein database. Mascot annotated mono- (A,C) and di-sialylated (B,D) core-1 O-linked glycopeptide spectra from two different peptide sequences.